Figure 5: Salmonella with the mgtB Pro 555, 556 Ala substitution produces higher MgtB protein amounts inside macrophages. | Scientific Reports

Figure 5: Salmonella with the mgtB Pro 555, 556 Ala substitution produces higher MgtB protein amounts inside macrophages.

From: Elongation factor P restricts Salmonella’s growth by controlling translation of a Mg2+ transporter gene during infection

Figure 5

Western blot analysis of crude extracts prepared from wild-type (14028s), the mgtB derivative with the Pro 555, 556 codons substituted by Ala codons (EN873) or an mgtB deletion mutant (EN481) Salmonella inside J774 A.1 macrophage-like cells 21 h after infection. The amounts of MgtB (A) and MgtC (B) proteins were determined by anti-MgtB or anti-MgtC antibodies. Anti-CorA antibodies were used to detect CorA proteins (C) as loading controls.

Back to article page