Figure 2: Knockdown of DLG5 promotes cell proliferation.
From: Loss of DLG5 promotes breast cancer malignancy by inhibiting the Hippo signaling pathway
(A) Immunoblot analysis of DLG5 expression in NC, MCF10A-shDLG5, MCF7-shDLG5 and MDA-MB-231-oxDLG5 cells. (B) The cells were counted at 24, 48 and 72 hrs. (C) NC and MCF7-shDLG5 cells were photographed every 8 hrs using a Live Cell Imaging System. Scale bar, 50 μm. (D,E) NC, MCF10A-shDLG5, MCF7-shDLG5 and MDA-MB-231-oxDLG5 cells were stained with PI and analyzed by flow cytometry (BD FACSCanto II) at 488 nm. (F,G) NC, MCF10A-shDLG5, MCF7-shDLG5 and MDA-MB-231-oxDLG5 cells were stained with BrdU-PerCP and analyzed by flow cytometry (FACSCanto II) at 488 nm. (H) The expression of cell proliferation-associated proteins, including cyclin D1, p21, p27 and p53, and the phosphorylation of ERK1/2 in NC, MCF10A-shDLG5, MCF7-shDLG5 and MDA-MB-231-oxDLG5 cells were analyzed by western blot.
