Figure 4: The functional analyses of DCs stimulated with OK-432. | Scientific Reports

Figure 4: The functional analyses of DCs stimulated with OK-432.

From: Interferon-α-inducible Dendritic Cells Matured with OK-432 Exhibit TRAIL and Fas Ligand Pathway-mediated Killer Activity

Figure 4

(a) DCs were incubated with FITC-dextran to assess pinocytotic activity (N = 6) and with DQ-ovalbumin to examine phagocytotic activity (N = 6). For each experiment, the results are expressed as the change in mean fluorescence intensity (MFI) and calculated by subtracting the MFI at 4 °C from that at 37 °C. (b) DCs were seeded into 24-well plates at a density of 2 × 105 cells/ml for 24 h, and the supernatants were subjected to enzyme-linked immunosorbent assay (ELISA) analysis to determine the levels of IL-12p70, IFN-γ, TNF-α, IL-10 and IL-6. A summary of the cytokine production data is shown (N = 8).

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