Figure 6: In mIFN-DCs, killing activity correlates with the expression of CD56 and is dependent on cell-to-cell contact. | Scientific Reports

Figure 6: In mIFN-DCs, killing activity correlates with the expression of CD56 and is dependent on cell-to-cell contact.

From: Interferon-α-inducible Dendritic Cells Matured with OK-432 Exhibit TRAIL and Fas Ligand Pathway-mediated Killer Activity

Figure 6

(a) The expression of CD56 on DCs is shown in terms of the mean fluorescence intensity (MFI). The expression of CD56 on mIFN-DCs and IFN-DCs was compared (N = 12). (b) Correlation between the expression of CD56 and mIFN-DC killing activity (N = 12). (c) Observed tumour killing mediated by IFN-DCs or mIFN-DCs in vitro. CFSE (green)-labelled K562 cells were incubated with PKH 26 (red)-stained IFN-DCs or mIFN-DCs at a ratio of 1:1. After an 18-h incubation, the cells were stained with DAPI. White arrows indicate dead cells stained with DAPI. White bar = 50 μm. (d) IFN-DCs or mIFN-DCs were incubated with K562 or Mia PaCa2 cells at a ratio of 50:1 and in the presence or absence of an insert membrane for 18 h (N = 6).

Back to article page