Figure 6: Proposed regulatory steps in EAL-PDE catalysed c-di-GMP hydrolysis.

Dimerisation induces a conformational change in the β5-α5 loop, allowing conserved aspartate residues to coordinate metal ions in the M1 and M2 position and c-di-GMP to bind. A third metal ion binds in the M3 position in the pGpG product complex.