Figure 3: Bigelovin caused G2/M cell cycle arrest and DNA damage. | Scientific Reports

Figure 3: Bigelovin caused G2/M cell cycle arrest and DNA damage.

From: Bigelovin triggered apoptosis in colorectal cancer in vitro and in vivo via upregulating death receptor 5 and reactive oxidative species

Figure 3

Cell cycle of two cancer cell lines treated with bigelovin (48 h) was evaluated by flow cytometry (a), and the results (24 h and 48 h bigelovin treatment) were quantified (b). The results were represented as the mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001 vs. medium control at the same time point. n = 3–4. Western blot analysis of apoptosis proteins, cell cycle regulatory proteins and DNA damage marker proteins in HT-29 (c) and HCT 116 (d) at indicated doses and quantitative data which were normalized with the protein level of actin and expressed as fold of control. The results were represented as mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001 vs. medium control at the same time point (n = 3–4).

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