Figure 2: 2D blue native/SDS-PAGE of an enriched J-dot fraction identifies high molecular weight complex containing PFE55GFP and PfHsp70x. | Scientific Reports

Figure 2: 2D blue native/SDS-PAGE of an enriched J-dot fraction identifies high molecular weight complex containing PFE55GFP and PfHsp70x.

From: Proteomic analysis of exported chaperone/co-chaperone complexes of P. falciparum reveals an array of complex protein-protein interactions

Figure 2

(A) Immunodetection of PFE55GFP following blue native separation. Three bands can be visualized, referring to distinct high molecular weight complexes (indicated by arrows). (B) Second dimension SDS separation followed by immunodetection demonstrates the presence of PfHsp70x and PFE55GFP in four distinct high molecular weight complexes (indicated by arrows). Inset shows higher contrast view. In (A,B) size markers in kDa. Antibodies used for immunodetection are indicated on right. Full-length blots are presented in the Supplementary Information file.

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