Table 1 Selected proteins identified by crosslink followed by co-IP using either anti-GFP or anti-PfHsp70x.

From: Proteomic analysis of exported chaperone/co-chaperone complexes of P. falciparum reveals an array of complex protein-protein interactions

Co-IP PFE55-GFP N = 7

ID

Description

UP

PC

UP control

PC control

Ratio IP/C

Previous ID(s)

Localisation

Exported N = 5

PF3D7_0501100.1/2

heat shock protein 40, type II (HSP40)

6

15,42

0

0,00

6/0

MAL5P1.12, PFE0055c

J-dots

PF3D7_0801000

Plasmodium exported protein (PHISTc, PfPHIST_0801), unknown function

3

4,10

0

0,00

3/0

PF08_0137

Unknown

PF3D7_1401100

DnaJ protein, putative

8

22,56

1

1,83

8,00

PF14_0013

Unknown

PF3D7_1407800

plasmepsin IV (PM4)

3

12,70

1

4,68

3,00

PF14_0075

FV

PF3D7_0831700

heat shock protein 70 (HSP70-x)

17

34,87

6

15,46

2,83

MAL7P1.228

J-dots

Signal peptide N = 1

PF3D7_1116800

heat shock protein 101 (HSP101)

5

9,27

0

0,00

5/0

PF11_0175

PV/PVM

TM Domain N = 1

PF3D7_1408100

plasmepsin III (HAP)

2

5,97

0

0,00

2/0

PF14_0078

FV

Co-IP PfHsp70x N = 34

ID

Description

UP

PC

UP control

PC control

Ratio IP/C

  

Exported N = 9

PF3D7_0831700

heat shock protein 70 (HSP70-x)

38

57,44

3

12,08

12,67

MAL7P1.228

J-dots

PF3D7_0402400

Plasmodium exported protein, unknown function (GEXP18)

6

36,61

0

0,00

6/0

MAL4P1.23, PFD0115c

Unknown

PF3D7_1016300

glycophorin binding protein (GBP)

6

46,48

0

0,00

6/0

PF10_0159

PV/RBCC

PF3D7_0501100.1/2

heat shock protein 40, type II (HSP40)

4

15,92

0

0,00

4/0

MAL5P1.12, PFE0055c

J-dots

PF3D7_1201000

Plasmodium exported protein (PHISTb), unknown function

4

6,28

0

0,00

4/0

2277.t00010, MAL12P1.10, PFL0050c

Unknown

PF3D7_0401800

Plasmodium exported protein (PHISTb), unknown function (PfD80)

2

4,46

0

0,00

2/0

MAL4P1.16, PFD0080c

Erythrocyte periphery/cytoskeleton

PF3D7_0721100

conserved Plasmodium protein, unknown function

2

9,84

0

0,00

2/0

PF07_0087

Unknown

PF3D7_1001400

alpha/beta hydrolase, putative

2

3,47

0

0,00

2/0

PF10_0018

Unknown

PF3D7_0801000

Plasmodium exported protein (PHISTc, PfPHIST_0801), unknown function

9

12,22

0

0,00

9/0

PF08_0137

Unknown

Signal peptide N = 18

PF3D7_1252100

rhoptry neck protein 3 (RON3)

9

5,60

0

0,00

9/0

2277.t00499, MAL12P1.496, PFL2505c

Rhoptry neck

PF3D7_1012200

conserved Plasmodium protein, unknown function

5

24,72

0

0,00

5/0

PF10_0119

Apical

PF3D7_1116800

heat shock protein 101 (HSP101)

4

6,51

0

0,00

4/0

PF11_0175

PV/PVM

PF3D7_1105800

conserved Plasmodium protein, unknown function

3

13,53

0

0,00

3/0

PF11_0069

Unknown

PF3D7_0302500

cytoadherence linked asexual protein 3.1 (CLAG3.1)

2

3,25

0

0,00

2/0

MAL3P1.5, PFC0120w

Apical/rhoptry

PF3D7_1015200.1/2

cysteine–tRNA ligase, putative (CysRS)

2

3,40

0

0,00

2/0

PF10_0149

Apicoplast

PF3D7_1116000

rhoptry neck protein 4 (RON4)

2

1,67

0

0,00

2/0

PF11_0168

Rhoptry neck

PF3D7_0930300

merozoite surface protein 1 (MSP1)

11

8,37

1

2,85

11,00

PFI1475w

Merozoite/ring surface

PF3D7_0207600

serine repeat antigen 5 (SERA5)

8

15,15

1

1,81

8,00

PF02_0072, PFB0340c

PV

PF3D7_1232100

60 kDa chaperonin (CPN60)

8

19,36

1

1,67

8,00

2277.t00309, MAL12P1.309, PFL1545c

Apicoplast

PF3D7_0827900

protein disulfide isomerase (PDI8)

5

18,63

1

3,42

5,00

MAL8P1.17

ER

PF3D7_0917900

heat shock protein 70 (HSP70-2)

19

36,35

4

7,36

4,75

PFI0875w

ER

PF3D7_0929400

high molecular weight rhoptry protein 2 (RhopH2)

25

25,04

6

6,39

4,17

PFI1445w

Rhoptry

PF3D7_1471100

exported protein 2 (EXP2)

4

14,98

1

3,83

4,00

PF14_0678

PVM

PF3D7_1222300

endoplasmin, putative (GRP94)

7

12,42

2

3,41

3,50

2277.t00214, MAL12P1.214, PFL1070c

ER

PF3D7_0905400

high molecular weight rhoptry protein 3 (RhopH3)

11

19,84

4

6,24

2,75

PFI0265c

Rhoptry

PF3D7_1129100

parasitophorous vacuolar protein 1 (PV1)

5

14,82

2

5,75

2,50

PF11_0302

PV

PF3D7_1454400

aminopeptidase P (APP)

17

29,09

8

13,26

2,13

PF14_0517

PV/FV

TM Domain N = 7

PF3D7_0811200

ER membrane protein complex subunit 1, putative (EMC1)

4

5,21

0

0,00

4/0

MAL8P1.105

J-dots

PF3D7_0220000

liver stage antigen 3 (LSA3)

3

2,95

0

0,00

3/0

PF02_0187, PFB0915w

Unknown

PF3D7_0823800

DnaJ protein, putative

3

8,40

0

0,00

3/0

PF08_0032

PV/RBCC

PF3D7_1037300

ADP/ATP transporter on adenylate translocase (ADT)

3

15,62

0

0,00

3/0

PF10_0366

J-dots

PF3D7_1105800

conserved Plasmodium protein, unknown function

3

13,53

0

0,00

3/0

PF11_0069

Unknown

PF3D7_1459400

conserved Plasmodium protein, unknown function

2

7,94

0

0,00

2/0

PF14_0567

Unknown

PF3D7_1311800

M1-family alanyl aminopeptidase (M1AAP)

12

17,51

1

2,58

12,00

MAL13P1.56

Unknown

Common to Co-IP GFP/PfHsp70x N = 4

ID

Description

       

PF3D7_0501100.1/2

heat shock protein 40, type II (HSP40)

       

PF3D7_0801000

Plasmodium exported protein (PHISTc, PfPHIST_0801), unknown function

       

PF3D7_0831700

heat shock protein 70 (HSP70-x)

       

PF3D7_1116800

heat shock protein 101 (HSP101)

       
  1. ID, PlasmoDB accession number; UP, unique peptides; PC, peptide coverage. Localization based on literature or Apiloc predictions. Proteins in italic were chosen for further study.
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