Figure 5: JP8 induces intracellular ROS-mediated apoptosis and autophagy. | Scientific Reports

Figure 5: JP8 induces intracellular ROS-mediated apoptosis and autophagy.

From: A novel ECG analog 4-(S)-(2,4,6-trimethylthiobenzyl)-epigallocatechin gallate selectively induces apoptosis of B16-F10 melanoma via activation of autophagy and ROS

Figure 5

(A,B) Co-treatment with NAC abolishes JP8-induced ROS accumulation in cancer cells. B16-F10 cells were treated with 20 μM JP8 for 12 h in the presence or absence of 2 mM NAC. Cells were stained with DHE and examined by flow cytometry. Bar graph represents the fold increase in ROS of three independent experiments expressed as the mean ± SEM, ***P < 0.001. (C,D) Co-treatment with NAC reduces JP8-induced apoptosis in B16-F10 cells. B16-F10 cells were treated with 20 μM JP8 for 12 h in the presence or absence of 2 mM NAC. Cell apoptosis was measured by annexin V/PI assays. The dot plots are representative and the bar graph is the mean ± SEM of three independent experiments, ***P < 0.001. (E,F) Western blot analysis of ROS-related proteins and autophagy marker LC3. B16-F10 cells were treated with 20 μM JP8 for 12 h in the presence or absence of 2 mM NAC. GAPDH was used as a loading control. Images are representatives and bar graphs are quantified results of three independent experiments expressed as the mean ± SEM, *P < 0.05, ***P < 0.001 compared to the JP8 treated group.

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