Figure 1: Schematic diagram of a dual-sided illumination for the light-sheet fluorescent microscope. | Scientific Reports

Figure 1: Schematic diagram of a dual-sided illumination for the light-sheet fluorescent microscope.

From: Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution

Figure 1

(a) 2-D optical layout highlights the achromatic doublets and the chamber for mounting the samples, and illustrates a pair of beams to provide dual-sided illumination to the samples. (b) 3-D layout of the imaging system reveals the individual optical components. M1-10: mirror; PH: pinhole; ND: neutral density filter; BE: beam expander; S: slit; CL1-2: cylindrical lens; L1-4: achromatic doublets; Ob1-2: objective.

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