Figure 1: Microinjection method and transient expression of GFP in C. semilaevis. | Scientific Reports

Figure 1: Microinjection method and transient expression of GFP in C. semilaevis.

From: Genome editing reveals dmrt1 as an essential male sex-determining gene in Chinese tongue sole (Cynoglossus semilaevis)

Figure 1

(A) Schematic drawing of microinjection. (1) A micropipette is passed through the chorion at an approximately 45-degree angle; (2) a droplet which is 1/10~1/5 of the cell volume is injected into the cell. (B) Transient expression of GFP in 2 dah C. semilaevis. (a) Control fish; (b) large magnification of frame area in (a); (c) transient expression of GFP in the muscles; (d) large magnification of frame area in (c) muscle fibres strongly expressing of GFP are indicated by arrows. Scale bar, 100 μm.

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