Figure 7: The Schedule of sampling.

Sexual differentiation happens at approximately 60-dph. The tissues for gonadal histology and gene expression studies were sampled at about approximately 1-yph. (I) 1-dph C. semilaevis. (II) 60-dph C. semilaevis. (III) 1-yph C. semilaevis. (A) Wild-type female C. semilaevis; (B) wild-type male C. semilaevis; (C) dmrt1-deficient male C. semilaevis.