Table 1 Generation of mutant mice by Cas9 protein:crRNA:tracrRNA complex injection.
Mouse gene | No. of mice at wean | Amino acid replacement | Mutation sequence | Correct knock-in efficiency |
|---|---|---|---|---|
SYNE1 | 17 | R257H | CGG- > CAT | 35% |
SYNE1 | 9 | W463D464- > AA | TGGGAC- > GCGGCC | 33% |
SYNE1 | 15 | E646K | GAG- > AAG | 53% |
NEXN | 6 | R274C | CGT- > TGT | 33% |
NEXN | 5 | G645DEL | GGA- > – | 40% |
TAX1BP3 | 14 | I33T | ATT- > ACT | 50% |
FLNC | 5 | S1625L | TCG- > TTG | 60% |
TLN | 3 | L432G | CTT- > GGT | 67% |
