Table 2 Sequences of crRNA, tracrRNA, and single-strand oligodeoxynucleotides used in this study.
Oligo name | Sequence |
|---|---|
R257H-crRNA | 5′-gcuggagguuucagaaaggaGUUUUAGAGCUAUGCUGUUUUG-3′ |
tracrRNA | 5′-AAACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAAC UUGAAAAAGUGGCACCGAGUCGGUGCU-3′ |
R257H-DNA | 5′-GCAGAAGTTTCTGGATGACTATTCTCGCTTTGAAGACTGGCTGGA GGTTTCAGAAAGGACATCTGCTTTCCCCAGTTCTTCCGGGGTGCTCT ATACAGTTGCCAAGGAGGAGCTGAAGAAG-3′ |
ALPK2-crRNA | 5′-gaggugacuugguauaagaaGUUUUAGAGCUAUGCUGUUUUG-3′ |
PXN-L-crRNA | 5′-ggucuugucuguucacucugGUUUUAGAGCUAUGCUGUUUUG-3′ |
PXN-R-crRNA | 5′-uggcugcucuugcuggaggaGUUUUAGAGCUAUGCUGUUUUG-3′ |
PXN-L-DNA | 5′-TTCATAGCTCTGCTGCTCACAGGATGCTCCTAGTAATGTTAGGTCTTG TCTGTTCACTCTGATAACTTCGTATAGCATACATTATACGAAGTTATAGGT AGGGTCTCCCTCCACAGCCCGGACTGTTGTGGAACTCACTACGTAGTAA GCCTCTTC-3′ |
PXN-R-DNA | 5′-ATCAGGAGGCACGGACACAGGGACAAAGAGCCCTGTGTTGATGGCTGCT CTTGCTGGAGGAATAACTTCGTATAGCATACATTATACGAAGTTATGGGTGT GTCCTGCTGGGCCCGTGGGATCAGGATCCCAAGCCTGAGAGGTCACTTGCC CCAG-3′ |
