Table 3 Primers used in this study.
Primer name | Sequence | GC% | Tm |
|---|---|---|---|
ALPK2-Forward(seq) | TTAGCATATCCCATTGTGCTCATC | 42 | 62 |
ALPK2-Reverse(seq) | AATTACTAGTGTGCTATACTGTCTAG | 35 | 61.7 |
SYNE1-R257H-F(wt) | TGGAGGTTTCAGAAAGGACGG | 52 | 61.2 |
SYNE1-R257H-F(mut) | TGGAGGTTTCAGAAAGGACAT | 43 | 57.5 |
SYNE1-R257H-F(rev) | GCTAATCTTCAATTAAAATCGTCACTG | 33 | 62.1 |
SYNE1-R257H-F(seq) | TCCCTGGGCGGTTTGGGTC | 68 | 63.6 |
OT1-Forward(seq) | GCTGAGTGCGACTCCGTCC | 68 | 63.6 |
OT1-Reverse(seq) | GGCTAGGTGATAAGGCTCTAGC | 55 | 64.2 |
OT2-Forward(seq) | GTCTTTGGTCTATGTGTGATCATG | 42 | 62 |
OT2-Reverse(seq) | CAAAGACAGGTGCAGAACAGG | 52 | 61.2 |
OT3-Forward(seq) | GTGTAAGACATTGTGTGAAGTCCT | 42 | 62 |
OT3-Reverse(seq) | CTGTTGCTCACAGAGTCGCCA | 57 | 63.2 |
OT4-Forward(seq) | ATGGCATTGAAACCTTGTCAGCATA | 40 | 62.5 |
OT4-Reverse(seq) | ACCAGCCAGTGTGTAACCAGC | 57 | 63.2 |
OT5-Forward(seq) | GACAGTCACCGACTTGTAAAGC | 50 | 62.1 |
OT5-Reverse(seq) | CCCTGCCACTGTGCTATCATG | 57 | 63.2 |
PXN-L-Forward(mut) | AACGTGGTCACGAGCCCAGC | 65 | 64.6 |
PXN-L-Reverse(mut) | GACCCTACCTATAACTTCGTATAATG | 38 | 62.9 |
PXN-R-Forward(mut) | CATTCTTCGACCTCAGTTGAGTAG | 46 | 63.6 |
PXN-R-Reverse(mut) | GACACACCCATAACTTCGTATAAT | 38 | 60.3 |
PXN-Forward(seq) | TCACGGGATGAGACGACATAGG | 55 | 64.2 |
PXN-Reverse(seq) | GTGTTCTGAGGACAGCAAGCG | 57 | 63.2 |
PXN-CRE-Forward | TCCAGTGAGACCATGTAGTCCC | 55 | 64 |
PXN-CRE-Reverse | GCCCTATCAAGGCAATACATCTTC | 46 | 63.6 |
