Table 1 Clustering of the gut microbiota of fecal samples from isolator- and IVC-housed mice.

From: Microbiota composition of simultaneously colonized mice housed under either a gnotobiotic isolator or individually ventilated cage regime

Unifrac distance matrix used for ANOSIM

Group

Hypothesis

p-value

R-value

Degree of separation

Unweighted

Isolator

P vs. F1 (all ages)

0.065(*)

0.20

low

P vs. F2 (all ages)

0.077(*)

0.15

low

F1 vs. F2 (4 weeks)

0.008*

0.21

low

F1 vs F2 (11 weeks)

0.636

0.03

none

Weighted

P vs. F1 (all ages)

0.135

0.11

none

P vs. F2 (all ages)

0.335

0.02

none

F1 vs. F2 (4 weeks)

0.002*

0.29

moderate

F1 vs. F2 (11 weeks)

0.491

0.00

none

Unweighted

IVCs

P vs. F1 (all ages)

0.002*

0.40

moderate

P vs. F2 (all ages)

0.015*

0.28

moderate

F1 vs. F2 (4 weeks)

0.002*

0.23

low

F1 vs F2 (11 weeks)

0.832

0.05

none

Weighted

P vs. F1 (all ages)

0.001*

0.37

moderate

P vs. F2 (all ages)

0.001*

0.37

moderate

F1 vs. F2 (4 weeks)

0.001*

0.50

moderate

F1 vs. F2 (11 weeks)

0.690

0.03

none

  1. Statistical tests between generation and age time points within the two housing systems based on weighted and unweighted UniFrac distance matrices. We considered a p-value > 0.05 and <0.08 as a tendency and is designated with (*). P-values < 0.05 are designated with * (ANOSIM with 999 permutations).
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