Figure 3: Cav1 rescues endothelial dysfunction due to lack of Sirt1. | Scientific Reports

Figure 3: Cav1 rescues endothelial dysfunction due to lack of Sirt1.

From: Sirtuin1 protects endothelial Caveolin-1 expression and preserves endothelial function via suppressing miR-204 and endoplasmic reticulum stress

Figure 3

Immunofluorescence for Cav1 in aortas (A) and mesenteric resistance arteries (MRA) (B) of mice with conditional deletion of endothelial Sirt1 (eSirt1−/−) and control Sirt1flx/flx mice, infected ex vivo with adenovirus expressing Cav1 (AD Cav1) or control adenovirus expressing LacZ (AD LacZ). Quantification of endothelial Cav1 is shown below immunofluorescence images. *p < 0.05 between eSirt1−/− + AD LacZ vs. Sirt1flx/flx + AD LacZ, Sirt1flx/flx + AD Cav1 and eSirt1−/− + AD Cav1. #p < 0.05 between Sirt1flx/flx + AD Cav1 vs. Sirt1flx/flx + AD LacZ, and eSirt1−/− + AD Cav1. Endothelium-dependent relaxation in thoracic aortas (C) and MRA (D) of eSirt1−/− and Sirt1flx/flx mice, infected ex vivo with AD Cav1 or AD LacZ. *p < 0.05 between eSirt1−/− + AD LacZ vs. Sirt1flx/flx + AD LacZ, Sirt1flx/flx + AD Cav1 and eSirt1−/− + AD Cav1. #p < 0.05 between Sirt1flx/flx + AD Cav1 vs. eSirt1−/− + AD LacZ, Sirt1flx/flx + AD LacZ, or eSirt1−/− + AD Cav1.

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