Figure 5: Chemotaxis of macrophages in the presence of niacin and kinase inhibitors. | Scientific Reports

Figure 5: Chemotaxis of macrophages in the presence of niacin and kinase inhibitors.

From: Activated niacin receptor HCA2 inhibits chemoattractant-mediated macrophage migration via Gβγ/PKC/ERK1/2 pathway and heterologous receptor desensitization

Figure 5

(A) RAW264.7 cells treated with LPS for 12 hs were pre-incubated with the ERK1/2 inhibitor UO126, the PI3K inhibitor Worttmannin, the p38 inhibitor SB203580, JNK inhibitor II and AKT inhibitor II for 15 min. Then the cells were cultured with 100 μM niacin for another 30 min. fMLF was used as a chemoattractant in all experiments. *p < 0.05; **p < 0.01, ***p < 0.001, compared with the cells cultured with 100 μM niacin and fMLF. (B) UO126 enhanced cell chemotaxis induced by fMLF. (C) EFG failed to inhibit the chemotaxis of HCA2/FPR1 co-transfected HEK293T cells. fMLF failed to induce chemotaxis of HCA2/FPR1/ERK2 co-transfected HEK293T cells.

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