Figure 5: Effect of orally administered CuII(atsm) on α-motor neurons, oxidative damage and astrogliosis in spinal cords of SOD1G93A mice.

(A) Quantitation of α-motor neurons per section in both ventral horn regions of spinal cord sections determined via cresyl violet staining. Only motor neurons with a diameter equivalent to 20 μm or greater were counted. (B) Abundance of oxidatively modified proteins determined using the OxyBlot assay in spinal cord tissue expressed relative to levels detected in sham-treated non-transgenic controls. Representative histology images for GFAP (C) and Iba-1 (D) immunoreactivity in spinal cord transverse sections. Data in (A and B) are presented as box (median ± 95% CI) and whisker (maximum and minimum) plots. P values represent statistically significant differences between mean values for indicated groups (one-way ANOVA with Tukey’s multiple comparisons test, n = 6 mice per treatment group). NS = not statistically different.