Figure 4: Copper-induced calcium variations in the PHA/PHB neurons.

(a,b) Cu2+ (20 mM) induced-calcium variations in the PHA/PHB neurons in wild-type worms, unc-13 mutants and unc-31 mutants. (a) Fluorescence changes (mean ± SEM); (b) Bar graphs. (c,d) Cu2+ (20 mM) induced-calcium variations in the PHA/PHB neurons in wild-type worms, osm-9 mutants and tax-4 mutants. (c) Fluorescence changes (mean ± SEM); (d) Bar graphs. Error bars indicate mean ± S.E.M.; ns, not significant, P > 0.05; **P < 0.01; ***P < 0.001; two-tailed paired Student t-test.