Figure 1: TPX2 depletion affects neurite elongation.

Dissociated hippocampal neurons were transfected with plasmid expressing non-targeting control shRNA (A) or Tpx2-targeting shRNA (B) at 2DIV and fixed at 5DIV. The insert shows a magnified image of the soma. Images were inverted and γ-corrected (γ = 0.05, in insert γ = 0.1) to enhance visualization. The scale bar presents 100 μm. Quantification of total neurite length per neuron (C), branch number per 200 μm neurite (D), and primary neurite number (E) of control and TPX2-depleted neurons. *p < 0.05 by two-tailed Student’s t-test. Error bars represent SEM from 3 independent experiments. More than 70 neurons were analyzed for each group from 3 independent experiments. (F) Schematic diagram of the neuron replating protocol. (G) Histogram of primary neurite number distribution of replated neurons. Neuron number of each group was normalized to total neuron number counted (non-targeting = 99, Tpx2-targeting = 92).