Figure 3: TPX2 localizes to the microtubules along the neurite shaft.
From: Ran-dependent TPX2 activation promotes acentrosomal microtubule nucleation in neurons
Representative images of PLA for TPX2 and β-III-tubulin in 1DIV (A) or 8DIV (B) dissociated hippocampal neurons. Dylight 488-conjugated secondary antibody was applied after PLA to stain β-III-tubulin antibody and to detect neurons (shown in green). DAPI stain was used to detect the nucleus (shown in blue). (C) PLA puncta were produced along the neurite shaft only when anti-TPX2 and anti-β-III-tubulin antibodies were both present. Alexa 405-conjugated secondary antibody was applied after PLA to stain β-III-tubulin antibody and to facilitate neurite visualization (shown in blue). All scale bars represent 10 μm.
