Figure 4: TPX2 depletion reduces microtubule emanating frequency at the tip and base of the neurite.
From: Ran-dependent TPX2 activation promotes acentrosomal microtubule nucleation in neurons
Cortical neurons were cotransfected with plasmids expressing EB3-mCherry and non-targeting or Tpx2-targeting shRNA before plating, incubated for 4 days before subjected to live cell imaging. (A) The image of a 4DIV cortical neuron expressing EB3-mCherry. The color windows indicated the region from which kymographs were generated. The scale bar represents 10 μm. (B) Kymographs of EB3-mCherry in indicated regions of the neurite. (C) Quantification of EB3-mCherry dynamics in control and TPX2-depleted neurons. *p < 0.05, two-tailed Student’s t-test. Error bars represent SEM from 3 independent repeats. At least 10 neurons were analyzed per condition per repeat.
