Figure 7: Importazole reduces microtubule emanating frequency at the tip and the base of the neurite.
From: Ran-dependent TPX2 activation promotes acentrosomal microtubule nucleation in neurons
Cortical neurons were transfected with plasmid expressing EB3-mCherry before plating, incubated for 4 days before subjected to live cell imaging in the presence of 15 μM importazole (IPZ). (A) Kymographs of EB3-mCherry in indicated regions of the neurite. (B) Quantification of EB3-mCherry dynamics in control (DMSO) or 15 μM IPZ (IPZ) treated neurons. EB3 dynamic properties before IPZ application (pre) and those after IPZ application (post) are compared. *p < 0.05, **p < 0.01, two-tailed Student’s t-test. Error bars represent SEM from 3 independent repeats. 6 neurons were analyzed per condition per repeat.
