Table 1 Analysis of inter-specific divergence between congeneric species and intra-specific variation of ITS2 and psbA-trnH.

From: Identification of crude drugs in the Japanese pharmacopoeia using a DNA barcoding system

Model

K2P distance

P distance

Nucleotides variation

DNA Barcode

ITS2

psbA-trnH

ITS2

psbA-trnH

ITS2

psbA-trnH

Sample size

285

33

285

33

285

33

Number of species

95

11

95

11

95

11

Average maximum intra-specific distance

0.0063 ± 0.0134

0.0034 ± 0.0058

0.0062 ± 0.0128

0.0033 ± 0.0057

0.0060 ± 0.0125

0.0031 ± 0.0054

Average intra-specific distance

0.0043 ± 0.0090

0.0022 ± 0.0038

0.0042 ± 0.0086

0.0022 ± 0.0038

0.0041 ± 0.0084

0.0020 ± 0.0036

Average minimum inter-specific distance

0.0221 ± 0.0412

0.0026 ± 0.0038

0.0206 ± 0.0364

0.0026 ± 0.0038

0.0196 ± 0.0354

0.0022 ± 0.0031

Average inter-specific distance

0.0393 ± 0.0447

0.0529 ± 0.0447

0.0365 ± 0.0386

0.0361 ± 0.0288

0.0351 ± 0.0377

0.0272 ± 0.0210

  1. Note: 1. Nucleotides variation = number of variation sites/full length after alignment. InDels were included.
  2. 2 Sequence statistics of psbA-trnH only retained the partial intergenic region when calculating.
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