Figure 8: Schematic diagramme summarising the changes in T. pseudonana metabolism in response to limitation by inorganic carbon and other resources.
Pathways are shown by grey circles and the component enzymes are shown as: 1, ribulose-1,5-bisphosphate carboxylase-oxygenase; 2, phosphoglycerate kinase; 3, fructose-bisphosphate aldolase; 4, fructose-1,6-bisphosphatase; 5, phosphoribulokinase; 6, triose-phosphate isomerase. The abbreviations used are: ADP, Adenosine diphosphate; APX ascorbate peroxidase; ATP, adenosine triphosphate; ATPSase, ATP synthase; BPGA, 1,3-bisphosphoglyceric acid; CA, carbonic anhydrase; Chl, chlorophyll; DHAP, dihydroxyacetone phosphate; F6P, fructose-6-phosphate; FBP, fructose-1,6-bisphosphate; Fd, ferredoxin; FNR, ferredoxin-NADP reductase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; G3P, glyceraldehyde-3-phosphate; hv, light; LCIP63, low CO2 inducible protein 63 kDa; LRPa, light-repressed protein a; NADPH, nicotinamide adenine dinucleotide phosphate (reduced); OPP, oxidative pentose phosphate pathway; PEP, phosphoenol pyruvate; PFK, phosphofructokinase; PGA, 3-phosphoglyceric acid; Pi, inorganic phosphate; PK, pyruvate kinase; PSI, photosystem I; PSII, photosystem II; Pyr, pyruvate; Ru5P, ribulose-5-phosphate; RuBP, ribulose-1,5-bisphosphate; Sti1, stress-inducible protein sti1; THP, transmembrane hypothetical protein. Changes in protein level in response to CO2-limitation are shown as increases (green arrows) or decreases (red arrows). Major interactions between the pathways and organelles are shown by grey lines. Also shown, as coloured boxes adjacent to proteins or pathways, are changes reported in the literature using proteomic or transcriptomic approaches in response to starvation by inorganic carbon (lilac) for T. pseudonana18; nitrogen (green) for T. pseudonana22,29, for P. tricornutum50; phosphorus (blue) for T. pseudonana25, for P. tricornutum19; silica for T. pseudonana (grey)29; iron (russet) for T. pseudonana29, for P. tricornutum46,48,49; fluctuating light (yellow-brown) for T. pseudonana26; temperature (turquoise) for T. pseudonana22,29. Responses for T. pseudonana (above the line) and for P. tricornutum (below the line) are denoted as an increase (+), decrease (−), both decrease and increase (±), no change (o). If the response is not commented on in the paper or if it is not obvious from Supplementary Information, no information is presented.
