Table 1 Changes in protein abundance and transcript levels on selected genes after T. pseudonana cells were switched from 20,000 to 50 ppm CO2.
Accession No. (NCBI) | Accession No. (Swiss Prot) | Gel | Spot ID | Annotation | Functional group | Protein fold change | RNA fold change |
|---|---|---|---|---|---|---|---|
220971674 | B8C995 | 3–11 | 435 | Translation elongation factor alpha | Protein synthesis | 4.11 | − |
220973545 | B8C239 | 3–11 | 431 | RL4e, ribosomal protein 4e 60S large ribosomal subunit | Protein synthesis | 3.86 | − |
220976737 | B8BTB5 | 4–7 | 422 | Transmembrane hypothetical protein (THP) | Carbon acquisition | 3.71 | 388 |
220970433* | B8CCH0* | 4–7 | 172 | LCIP63 | Unknown function | 3.60 | 409 |
220969212 | B8CGE1 | 3–11 | 549 | Stress-inducible protein sti1 (BLAST) | Stress | 3.42 | 2.27 |
220976737 | B8BTB5 | 3–11 | 696 | Transmembrane hypothetical protein (THP) | Carbon acquisition | 3.41 | 388 |
209583601 | B5YLS7 | 4–7 | 368 | Triose-phosphate isomerase | Carbohydrate metabolism | 3.20 | − |
220978199 | B8BRE6 | 4–7 | 378 | Light-repressed protein a | Protein synthesis | 2.90 | 1.83 |
220969257 | B8CFA9 | 4–7 | 380 | Ascorbate peroxidase | Stress | 2.61 | − |
220970433* | B8CCH0* | 3–11 | 408 | LCIP63 | Unknown function | 2.40 | 409 |
220972037 | B8C7S8 | 4–7 | 585 | Hypothetical protein THAPSDRAFT_7881 | Unknown function | 2.32 | − |
220978087 | B8BQS1 | 3–11 | 926 | Protein with CP12 domain (BLAST) | Photosynthesis | 2.29 | 2.15 |
220978087 | B8BQS1 | 4–7 | 425 | Protein with CP12 domain (BLAST) | Photosynthesis | 2.23 | 2.15 |
220975793 | B8BX06 | 3–11 | 627 | 14–3–3-like protein | Degradation & signalling | 2.13 | − |
220969044 | B8CGL9 | 4–7 | 317 | Ferredoxin-NADP reductase | Photosynthesis | 2.06 | − |
220974564 | B8BZK1 | 4–7 | 342 | Phosphomannomutase | Carbohydrate metabolism | 2.06 | − |
220976367 | B8BU33 | 3–11 | 875 | Fucoxanthin-chlorophyll a/c light-harvesting protein | Photosynthesis | 1.99 | − |
220969044 | B8CGL9 | 3–11 | 541* | Ferredoxin-NADP reductase | Photosynthesis | 1.74 | − |
220969727 | B8CEN5 | 4–7 | 389 | Glycoprotein fp21 (BLAST) | Unknown function | 1.67 | − |
589908182 | W8VYH0 | 4–7 | 420 | Delta carbonic anhydrase | Carbon acquisition | 1.65 | − |
220974490 | B8BZ41 | 4–7 | 266 | Phosphofructokinase | Carbohydrate metabolism | −1.45 | − |
224011563 | B5YLQ5 | 3–11 | 366 | Mitochondrial chaperonin | Chaperone | −1.51 | − |
125987721 | A0T0R6 | 4–7 | 207 | ATP synthase CF1 beta chain | Energy/ATP | −1.54 | − |
209586260 | B5YN92 | 3–11 | 914 | Phosphoglycerate kinase (BLAST) | Photosynthesis | −1.55 | − |
220968997 | B8CGK1 | 4–7 | 588 | Glutamine synthetase (BLAST) | Nitrogen metabolism | −1.56 | − |
220970599 | B8CCE1 | 4–7 | 322 | Pseudouridylate synthase | Protein synthesis | −1.57 | − |
125987749 | A0T0 × 9 | 3–11 | 684 | 30S ribosomal protein S3, chloroplastic | Protein synthesis | −1.58 | − |
209586260 | B5YN92 | 3–11 | 915 | Phosphoglycerate kinase (BLAST) | Photosynthesis | −1.60 | − |
220976316 | B8BTR4 | 4–7 | 515 | Transketolase | Carbohydrate metabolism | −1.61 | − |
220969226 | B8CGI1 | 3–11 | 672 | Demethylmenaquinone methyltransferase (BLAST) | Secondary metabolism | −1.61 | − |
220968997 | B8CGK1 | 4–7 | 590 | Glutamine synthetase (BLAST) | Nitrogen metabolism | −1.61 | − |
220968642 | B8LCI4 | 4–7 | 593 | Phosphoglycerate dehydrogenase (BLAST) | Nitrogen metabolism | −1.63 | − |
220975991 | B8BY55 | 4–7 | 199 | S-adenosylmethionine synthetase | Nitrogen metabolism | −1.65 | − |
220970584 | B8CCA0 | 4–7 | 269 | Aspartate-ammonia ligase | Nitrogen metabolism | −1.65 | − |
209586260 | B5YN92 | 3–11 | 461 | Phosphoglycerate kinase (BLAST) | Photosynthesis | −1.68 | − |
220973284 | B8C635 | 3–11 | 369 | Heat shock protein 70 | Chaperone | −1.69 | − |
220969617 | B8CET1 | 3–11 | 921 | PEFG, plastid translation factor EF-G (BLAST) | Protein synthesis | −1.70 | − |
118411188 | A0T0 × 0 | 3–11 | 427 | 60 kDa chaperonin | Chaperone | −1.71 | − |
125987721 | A0T0R6 | 3–11 | 860 | ATP synthase CF1 beta chain | Energy/ATP | −1.76 | − |
220977307 | B8BT02 | 4–7 | 233 | Aspartate aminotransferase | Nitrogen metabolism | −1.77 | − |
220970665 | B8CCS6 | 3–11 | 438 | Serine hydroxymethyltransferase | Nitrogen metabolism | −1.80 | − |
118411103 | A0T0N5 | 4–7 | 541 | Rubisco small subunit | Photosynthesis | −1.82 | − |
209583569 | B5YLM0 | 4–7 | 182 | Histidinol dehydrogenase | Nitrogen metabolism | −1.82 | − |
220977520 | B8BQU2 | 3–11 | 919 | Glyceraldehyde-3-phosphate dehydrogenase precursor | Photosynthesis | −1.88 | − |
220970292 | B8CDB3 | 3–11 | 857 | Geranylgeranyl reductase (BLAST) | Secondary metabolism | −1.92 | − |
220972213 | B8C6C6 | 3–11 | 849 | Mitochondrial ATPase, inner membrane | Energy/ATP | −1.98 | − |
118411103 | A0T0N5 | 4–7 | 542 | Rubisco small subunit | Photosynthesis | −2.00 | − |
220968222 | B8LDL7 | 4–7 | 592 | N-acetyl-glutamate-gamma-semialdehyde dehydrogenase | Nitrogen metabolism | −2.07 | − |
220971973 | B8C8K9 | 4–7 | 526 | RS1, ribosomal protein 1, partial | Protein synthesis | −2.07 | − |
209585841 | B5YMV8 | 3–11 | 350 | Regulation, link with ATP synthase (BLAST) | Energy/ATP | −2.19 | − |
125987721 | A0T0R6 | 3–11 | 415 | ATP synthase CF1 beta chain | Energy/ATP | −2.22 | − |
220977926 | B8BT70 | 3–11 | 847 | Oxidoreductase | Carbohydrate metabolism | −2.28 | − |
220977307 | B8BT02 | 4–7 | 524 | Aspartate aminotransferase | Nitrogen metabolism | −2.29 | − |
220977307 | B8BT02 | 4–7 | 226 | Aspartate aminotransferase | Nitrogen metabolism | −2.56 | − |
209586260 | B5YN92 | 3–11 | 848 | Phosphoglycerate kinase (BLAST) | Photosynthesis | −2.66 | − |
220972213 | B8C6C6 | 3–11 | 897 | Mitochondrial ATPase, inner membrane | Energy/ATP | −3.43 | − |
