Figure 7: Immunolocalization of phosphorylated p38 MAPK/JNK in the EC of mhAPP mice. | Scientific Reports

Figure 7: Immunolocalization of phosphorylated p38 MAPK/JNK in the EC of mhAPP mice.

From: Entorhinal Cortex dysfunction can be rescued by inhibition of microglial RAGE in an Alzheimer’s disease mouse model

Figure 7

(A) NeuN (neuronal) labeled cells (left column) and phosphorylated-p38 fluorescent staining (p-p38, middle columns). Representative images (20×) show that p-p38 fluorescent signal was increased in EC superficial layers of 2 month old mhAPP mice (middle raw) respect to age-matched wild-type (upper panels). Phospho-p38 immunofluorescence co-localizes with the neuronal marker NeuN (merge in right column and enlarged neuron indicated by the arrow in the insert). (B) NeuN (neuronalmarker) labeled cells (left column) and phosphorylated-JNK (p-JNK) immunoreactivity (middle columns). Cells in the right column (merge) are double-labeled forNeuN and p-JNK. Phosphorylated form of JNK was rarely observed in 6 month old WT mice (upper raw). Representative images (20×) show that p-JNK immunofluorescence is increased in the layer II/III of mhAPP EC (middle raw) respect to age-matched WT (upper raw). The pattern of p-JNK immunolabeling strongly co-localize with the neuronal marker NeuN (merge in right column and insert of a neuron indicated by arrow at higher magnification). Scale bar = 50 μm.

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