Figure 1: Differentiation of HAP_R01 and SBA_R01.

(a) Chemical structures of HAP and SBA reference compounds. (b) Activity of HAP and SBA compounds. Shown are mean IC₅₀ values of biochemical quenching assay, mean EC₅₀ values of HepG2.2.15 antiviral assay and mean CC₅₀ values of cytotoxicity experiment (±standard deviation). Each is from three independent experiments. (c) Electrophoresis of core particles in HepG2.2.15 cell lysate. Both tested compounds are effective in reducing encapsidated DNA. HAP_R01-treated cells show concentration-dependent reduction of capsid level on a native agarose gel and core protein level on a denatured gel. In contrast, SBA_R01 does not diminish either capsid level or core protein level at non-cytotoxic concentrations. The cropped DNA and native gels are shown here for clarity. The full-length blots for the DNA and native gels are presented in Supplementary Fig. S13. Actin is the loading control. (d) Electron micrographs of compound-treated core protein assembly at the 1:1 dimer-to-compound ratio. Left: capsid control induced by 250 mM NaCl. Middle: 5 μM core protein dimer incubated with 5 μM HAP_R01. Right: 5 μM core protein dimer incubated with 5 μM SBA_R01. Black scale bar indicates 50 nm.