Figure 2

Azo-carmine and aniline blue staining of collagen fibres in paraffin sections from adjuvant control (a,d,g), low grade (b,e,h), and severe EAEO (c,f,i) mouse testes at 30 (a–c), 50 (d–f) and 80 (g–i) days after first immunisation. An increase in collagen fibres is visible in low grade EAEO in the areas with lymphocytic infiltrates. A strong peritubular fibrotic response is detectable in EAEO mouse testes at 50 (f) and 80 (i) days after the first immunisation. Scale bars represent 100 μm.