Figure 3 | Scientific Reports

Figure 3

From: Testicular activin and follistatin levels are elevated during the course of experimental autoimmune epididymo–orchitis in mice

Figure 3

Double staining for CD206 (AlexaFluor546, orange) (a,e,i,m) and the macrophage marker F4/80 (AlexaFluor488, green) (b,f,j,n) as well as MHC class II single staining (d,h,l,p) in testicular cryosections from untreated (a–d), adjuvant control (e–h), low grade EAEO (i–l) and severe EAEO (m–p) mice. Nuclei were counterstained with DAPI (blue). Under non-inflammatory conditions, co-localized CD206 and F4/80 positive macrophages (c,g) and MHC class II positive cells (d–h) were present in low numbers in the interstitial space. An accumulation of double positive F4/80 and CD206 macrophages and MHC class II positive cells was observed in inflamed low grade (k,l, respectively) and severe (o,p, respectively) 50 day EAEO testis, with a higher number of F4/80 positive only (CD206-negative) cells. In low grade 50 day EAEO testis (k), the accumulation of macrophages was present in areas with reduced tubule diameter, whereas in severe 50 day EAEO testis (m–p) macrophages and MHC class II positive cells were more evenly distributed in the interstitial space. Scale bars represent 100 μm.

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