Figure 6: Upregulation of p62 reduces sensitivity of vitiligo melanocytes to H2O2-induced oxidative stress.
From: Dysregulated autophagy increased melanocyte sensitivity to H2O2-induced oxidative stress in vitiligo
(A) PIG3V cells were stably infected with or without the Nrf2 shRNA, then cells were transfected with or without pCMV-MCS-p62 for 24 hours and then treated with H2O2 (0.5 mM) for 24 hours, the level of apoptosis was detected by flow cytometry assay. (B) The level of apoptosis are presented as the mean ± SD, **p < 0.01. (C) PIG3V cells were stably infected with or without the Nrf2 shRNA, then cells were transfected with pCMV-MCS-p62 for 24 hours and then treated with H2O2 for 24 hours, the MMP was measured by flow cytometry assay. (D) Quantification of MMP are presented as the mean ± SD, **p < 0.01. (E) PIG3V cells were stably infected with or without the Nrf2 shRNA, then cells were transfected with pCMV-MCS-p62 for 24 hours and then treated with H2O2 for 24 hours, the intracellular ROS level was quantized by flow cytometry assay. (F) Quantification of intracellular ROS level are presented as the mean ± SD, **p < 0.01.
