Figure 2: CK2 activity in CK2α/α’ knockout cells.

CK2 activities were analyzed in WT and in CK2α/α’ knockout clones by in gel assay (A) and phosphorylation of exogenous substrate (GST-HDGF) in cell lysates (B). A 30 μg of lysate proteins were loaded on a polyacrylamide gel containing the CK2-substrate β-casein and CK2α/α’ activity was detected as detailed in Methods. (B) Cellular kinase activity of CK2 was tested in vitro in a phosphorylation medium containing lysate proteins, [γ³³P]ATP and the protein substrate GST-HDGF as described in Methods. 100 nM CX-5011 was added where indicated.