Figure 3: Immunization with adjuvanted REG protects cotton rats against RSV challenge.
Cotton rats were challenged i.n. with 1 × 105 pfu of RSV-A2 at 21 days after the second immunization. Two days post virus challenge, nose (A) and lung (B) tissues were collected and homogenized as described in materials and methods, and viral loads were determined by plaque assay. (C,D) Relationships between the PRNT neutralization titers in each individual post- vaccination sera from all vaccinated-viral challenged animals (Gps B-F) and either nasal viral loads (C) or lung viral loads (D) 2 days post-challenge with RSV-A2 with Spearman’s correlations. (E-H) Relationships between the total anti-G antibody binding to REG (E,F) or RMG (G,H) of individual post- vaccination sera from REG-vaccinated animals (Gps C and D) in SPR and nasal viral load (E and G) or lung viral load (F and H) at 2 days post-challenge with RSV-A2 with Spearman’s correlations. Total antibody binding to REG or RMG protein is represented in resonance units and viral loads are represented as pfu per gram tissue weight. The symbol colors correspond to the groups shown in Fig. 2.
