Figure 7
From: Flavichalasines A–M, cytochalasan alkaloids from Aspergillus flavipes
(A) The viability of HL60 cells after treated with compounds 6 and 14 were determined by CCK-8 kit. Mean ± SD of three independent experiments. (B) Cell apoptosis was determined by Annexin V-FITC and PI staining using flow cytometric analysis. (C) Columns, means of three independent FACS assay for apoptosis; bars, SD, P value were calculated by two-tailed student’s t-test. (D) Western blot analysis for the apoptosis marker PARP and cleaved-caspase-3, β-Actin was used as a loading control.
