Figure 2: A Notch signaling-dependent pathway may have modulated Th1, Th17, and Treg cell differentiation in chronic AA patients who received a MSC transfusion. | Scientific Reports

Figure 2: A Notch signaling-dependent pathway may have modulated Th1, Th17, and Treg cell differentiation in chronic AA patients who received a MSC transfusion.

From: In patients with chronic aplastic anemia, bone marrow–derived MSCs regulate the Treg/Th17 balance by influencing the Notch/RBP-J/FOXP3/RORγt pathway

Figure 2

(A) Q-PCR analyses of Notch, Notch2, Notch3, and Notch4 expression in the PBMCs of patients treated with MSCs. (B) Q-PCR analyses of Dll1, Dll2, and Dll3 expression in the PBMCs of patients treated with MSCs. a (C) Q-PCR analyses of Jaggedl and Jagged2 expression in the PBMCs of patients treated with MSCs. (D) Q-PCR analyses of RBP-J, RORγT, and Foxp3 expression in the PBMCs of patients treated with MSCs. (E) Western blot analyses of Notchl, Notch2, Dll1, Jaggedl, RBP-J, RORγT, and Foxp3 expression in the PBMCs of patients. GAPDH was used as a loading control. (F) Densitometry plot of results shown in Fig. 2E. The relative expression levels were normalized to GAPDH. Data represent the mean ± standard error (n = 3). Data represent the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

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