Figure 5: Knockdown of FMNL3 alters FMNL3 and EMT marker gene expression in CNE2 cells. | Scientific Reports

Figure 5: Knockdown of FMNL3 alters FMNL3 and EMT marker gene expression in CNE2 cells.

From: High FMNL3 expression promotes nasopharyngeal carcinoma cell metastasis: role in TGF-β1-induced epithelia-to-mesenchymal transition

Figure 5

CNE2 cells were transfected with FMNL3-specific siRNA (siFMNL3 oligo 1), control siRNA (siNC), or no siRNA (MOCK) and then treated with or without 10 ng/ml TGF-β1 for 48 h. The cells were harvested and analysed for FMNL3, E-cadherin, and Vimentin mRNA and protein expression by (A) Immunofluorescent staining and qRT-PCR (B). For immunofluorescent staining, images of E-cadherin and Vimentin photographed from the same field were visualized with FITC- or TRITC-coupled IgGs. For qRT-PCR and western blotting, GAPDH was used as a loading control. **p < 0.01, #p < 0.05 and Δp < 0.05.

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