Figure 2: Photos of the electroporation station for the HiCEP microsystem. | Scientific Reports

Figure 2: Photos of the electroporation station for the HiCEP microsystem.

From: High-throughput in situ cell electroporation microsystem for parallel delivery of single guide RNAs into mammalian cells

Figure 2

(A) Front overview of the station. An inverted microscope was set up in a custom-built glovebox, in which the humidity was maintained to prevent the droplet evaporation and the condition was sterilized using UV lamps for cell manipulation. (B) Modified microscope stage with a vacuum chip holder and a chip stage for the EP and the SM chips, respectively. (C) Expanded view of the microchips on the station. The EP chip was held by the vacuum chip holder with electrode side facing down, while the SM chip was position onto the chip stage. Both of the microchips were aligned using a custom-built micromanipulator with the aid of the CCD cameras.

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