Figure 5: Optimization and evaluation of cell electroporation performed on the HiCEP. | Scientific Reports

Figure 5: Optimization and evaluation of cell electroporation performed on the HiCEP.

From: High-throughput in situ cell electroporation microsystem for parallel delivery of single guide RNAs into mammalian cells

Figure 5

(A) Image of the microwells with various concentrations of Rhodamine B delivered using the spot-cover method. A linear relationship between the Rhodamine B concentration and the fluorescence intensity was obtained with an R2 equal to 0.969 (n = 8). (B) Optimization of the amplitude of the electric pulse applied to the electrodes. At the voltage of 80 V, the transfection efficiency reached the maximum value of 58.1%, while the cell viability is slightly decreased with the increase of the voltage. (C) High-throughput electroporation of HeLa cells. The average transfection efficiency is 58.4 ± 9.3%, and the percentage of the dead cells is 2.0 ± 0.9% (n = 8). (D) Flow cytometry result of the transfected cells recovered from the SM chip after electroporation. The overall electroporation efficiency is 65.5%, and the percentage of dead cells is 4.3%. All data are shown as the mean ± standard deviation.

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