Figure 7: Selective delivery of single guide RNA into 293T cells on the HiCEP system. | Scientific Reports

Figure 7: Selective delivery of single guide RNA into 293T cells on the HiCEP system.

From: High-throughput in situ cell electroporation microsystem for parallel delivery of single guide RNAs into mammalian cells

Figure 7

(A) Typical fluorescent micrographs of Cas9-expressing 293T cells transfected with different sgRNAs in parallel. Cells with pGR-Hygro-P plasmids (repaired) demonstrated a higher level of GFP fluorescent intensities than those of cells with pGR-Hygro-N (mutant), illustrating the effectiveness of the CRISPR/Cas9 system on the chip. (B) The difference of fluorescence intensities between these two groups is more than 4 times (n = 8, two-tailed, unpaired t-test, p < 0.001). No change was obtained before and after electroporation in the mutant group. (C) Sanger sequencing results of the repaired and the mutant sequences. Mixed sequences were obtained from the repaired group, suggesting an indel was successfully introduced into the plasmids by the CRISPR/Cas9 system.

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