Figure 7: Synaptic expression of endogenous β-neurexins is decreased in J20 APP mice.
(a) Representative immunoblots of neurexins (NRXs) in synaptosomes from the hippocampus and from the cerebral cortex of J20 APP mice and wild-type (WT) littermates at 6 months of age. The labels 1, 2, and 3 indicate samples from different mice. Full gel blots for the cropped blots (a) are shown in the Supplementary Fig. 4. (b) Quantification of synaptic expression of β-NRXs (bands indicated by a lower right square bracket in (a) and α-NRXs (bands indicated by upper right square bracket in (a) normalized to β-actin protein expression in synaptosomes from the hippocampus and the cortex, expressed relative to WT. n = 5 samples per genotype for hippocampus, with each n representing pooled hippocampi from two mice. n = 6 samples per genotype for cortex, with each n representing a cortex from one mouse. Unpaired t tests, #P < 0.05 for β-NRXs and P = 0.15 for α-NRXs in hippocampus, and **P < 0.001 for β-NRXs and P = 0.31 for α-NRXs in cortex. n.s., not significant. Data are presented as mean ± SEM.
