Figure 1: Panx1^−/− mice are resistant to SNI-induced mechanical hypersensitivity.

(A) SNI and CCI models. (Image adapted from⁵⁵) (B) Absence of mechanical hypersensitivity in Panx1^+/+ mice undergoing sham surgery (n.s., p > 0.46 by two-way RM ANOVA). (C) Wild-type littermates underwent unilateral SNI and developed mechanical hypersensitivity in the operated limb. In contrast, Panx1^−/− mice were protected from mechanical hypersensitivity. (D) Summary data (for C) is the mean (±SEM) of the threshold value for each mouse, averaged over the period from POD7-28 (depicted by the shaded region in the time series). (E,F) Like SNI, mechanical hypersensitivity following CCI is completely absent in Panx1^−/− mice while wild-type littermates experience robust hypersensitivity. In this and all other figures showing the time course of mechanical or thermal sensitivity, two-way RM ANOVA was used for statistical analysis, with Tukey’s test for pairwise comparisons: ^#p < 0.05 versus day 0; *p < 0.05 for contralateral versus ipsilateral paw. For all figures showing summary data, the averaged value from each mouse from POD7-28 was treated as a single data point in calculating group means that were compared by two-way RM ANOVA; *p < 0.05 between indicated groups, with Sidak’s test used for pairwise comparisons.