Figure 6: The PSR chromosome is devoid of H3K27me1 but not H3K9me2,3 and H4K20me1. | Scientific Reports

Figure 6: The PSR chromosome is devoid of H3K27me1 but not H3K9me2,3 and H4K20me1.

From: A ‘selfish’ B chromosome induces genome elimination by disrupting the histone code in the jewel wasp Nasonia vitripennis

Figure 6

(A) chromosome spread showing the five normal chromosomes in the N. vitripennis genome (top of panel) and the PSR chromosome (bottom of panel, in white box). Right panels show a higher magnification of the PSR chromosome from left panel, hybridized with a DNA FISH probe that highlights a satellite sequence that is present across most of the larger arm (green arrowhead) and in a small region on the shorter arm (white arrowhead). (B,C,D) PSR stained for the three histone marks H3K9me2,3, H3K27me1, and H4K20me1, respectively. Middle and far right column are higher magnifications of PSR, with the middle panels depicting the histone mark and PSR FISH probe. The far right panels show the same images without the PSR FISH probe in order to clearly visualize whether PSR contains a given histone mark. PSR is circumscribed (white line) in middle and bottom rows for clarity. Scale bar equals 12 μM.

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