Figure 4: Viral spread in cultured corneas. | Scientific Reports

Figure 4: Viral spread in cultured corneas.

From: Cultured corneas show dendritic spread and restrict herpes simplex virus infection that is not observed with cultured corneal cells

Figure 4

(a) Representative images of porcine corneas showing the spread of HSV-1 17-GFP virus at indicated virus inoculums and times post-infection. Scale bar for all images: 1000 μm. (b) qRT-PCR analysis of viral gD transcripts from porcine corneas at indicated times are shown. Relative levels to GAPDH are shown. Two-way ANOVA was performed using Tukey’s correction. 24 hpi: UI vs 105, 106 vs 107: ns; UI vs 106, 105 vs 106, 105 vs 107: **P < 0.01; UI vs 107: ***P < 0.001. 48 hpi: UI vs 106, 106 vs 107, 105 vs 106, UI vs 105, UI vs 107, 105 vs 107: ****P < 0.0001 (c) qRT-PCR analysis of intracellular cytokine transcripts from porcine corneas are shown. Relative levels to GAPDH are shown. Two-way ANOVA was performed using Sidak’s correction. TNF-α: 24 hpi: UI vs 106, 106 vs 107, 105 vs 106: ****P < 0.0001. UI vs 105, UI vs 107, 105 vs 107: ns; 48 hpi: UI vs 106, 106 vs 107, 105 vs 106, UI vs 105, UI vs 107, 105 vs 107: ****P < 0.0001. IFN-α: 24 hpi: UI vs 106, 106 vs 107, 105 vs 106, UI vs 105, UI vs 107, 105 vs 107: ns. 48 hpi: UI vs 106, UI vs 107, 106 vs 107: ns. UI vs 105, 105vs 106, 105 vs 107: ****P < 0.0001. IFN-β: 24 hpi: UI vs 106, 105 vs 106, 105 vs 107, 106 vs 107: ns. UI vs 105, **P < 0.01. UI vs 107, ***P < 0.001. 48 hpi: UI vs 107, 106 vs 107: ns. UI vs 105, 105 vs 107: ****P < 0.0001. UI vs 106: **P < 0.01. 105 vs 106: ***P < 0.001.

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