Figure 4: Characteristics of murine primary-cultured microglia preconditioned by oxygen-glucose deprivation (OGD).
From: Microglia preconditioned by oxygen-glucose deprivation promote functional recovery in ischemic rats
(A,B) The levels of secretory vascular endothelial growth factor (VEGF), brain derived neurotrophic factor (BDNF) (A) and matrix metalloproteinase-9 (MMP-9) (B) from conditioned media of murine primary-cultured microglia subjected to normoxia (norm) or OGD (N = 6–8 each). **P < 0.01. (C) The levels of secretory anti-inflammatory cytokines such as transforming growth factor-β (TGF-β) and interleukin-10 (IL-10), and pro-inflammatory cytokines such as IL-6, tumour necrosis factor-α (TNF-α), and IL-1β from conditioned media of murine primary-cultured microglia subjected to normoxia (norm) or OGD (N = 6 each). **P < 0.01. (D) The ratio of TGF-β per TNF-α, which reflects microglial polarization after normoxia and OGD preconditioning (N = 6 each). The increase in the ratio of TGF-β per TNF-α reveals the polarization of M2 microglia after OGD preconditioning. **P < 0.01.
