Figure 1: Flow cytometry and lipid analysis of Bone marrow derived macrophages (BMMs) and dendritic cells (BMDCs). | Scientific Reports

Figure 1: Flow cytometry and lipid analysis of Bone marrow derived macrophages (BMMs) and dendritic cells (BMDCs).

From: Transient Cannabinoid Receptor 2 Blockade during Immunization Heightens Intensity and Breadth of Antigen-specific Antibody Responses in Young and Aged mice

Figure 1

(a) Purity of BMMs were analyzed using anti-F4/80-PE-Cy7 and anti-CD11b-BV785 antibodies. Matured and fully differentiated BMMs are double positive for F4/80 and CD11b (top right quadrant). (b) BMDCs were stained with anti-CD11c-APC and anti-CD11b-BV785 antibodies. Matured and fully differentiated BMDCs are double positive for CD11c and CD11b (top right quadrant). (c,d) Lipid analysis of BMMs and BMDCs activated overnight with 0.1 μg/mL LPS. Bar graphs are expressed as mean lipid amount ± SEM (n = 3). Statistical differences were determined by nonparametric Mann-Whitney U test, ∗P < 0.05, compared to No LPS control.

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