Figure 6: Transient CBR2 blockade during vaccination increases the intensity and breadth antigen-specific antibody responses in young and aged mice.

8 weeks old and 20 month old mice were immunized with P-11 mixed- antigen vaccine. Animals were vaccinated at day 0 and given a boost at day 14 with endpoint at day 28. Serum collected at day 28 post-vaccination was probed on a custom microarray printed with constituent proteins of P-11 vaccine. (a,c) Bar graphs representing mean signal intensities of serum antigen-specific IgG antibodies (b,d) Scatter plots representing number of reactive antigen-specific responses. Bar graphs and scatter plots expressed as mean ± SEM (n = 5). Statistical analysis performed by nonparametric Mann-Whitney U test, *p < 0.05 or **p < 0.01, all samples compared to vehicle control.