Figure 7: The effect of CB2R modulation on the expression of immunomodulatory genes in young and aged mice. | Scientific Reports

Figure 7: The effect of CB2R modulation on the expression of immunomodulatory genes in young and aged mice.

From: Transient Cannabinoid Receptor 2 Blockade during Immunization Heightens Intensity and Breadth of Antigen-specific Antibody Responses in Young and Aged mice

Figure 7

To evaluate the effect of CBR2 modulation on expression of immunomodulatory genes in vaccinated young and aged mice, splenocytes from mice treated with either vehicle, 10 mg/kg AM630 or 3 mg/kg AM1241 from days 0–4 post-immunization were harvested and single-cell suspension prepared at end point of vaccination at day 28 post-vaccination. QPCR was performed on splenocytes using specific primers to determine the levels of immunomodulatory genes (a,g) MHC II, (b,h) CXCR5, (c,i) CCR7, (d,j) L-Selectin, (e,k) SWAP70 and (f,l) Connexin43. Gene expression levels were normalized to GAPDH. Results are expressed as mean ± SEM; n = 6–8/group. Statistical analysis conducted by nonparametric Mann-Whitney U test, *p < 0.05 or **p < 0.01, all samples compared to vehicle control.

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