Figure 5: Effect of SNPs on the activation of autophagy.
From: New findings of silica nanoparticles induced ER autophagy in human colon cancer cell
The merge of the Cyto-ID fluorescence dye and Hoechst 3342 in HCT-116 cells treated with DMSO (a), 12.5 μM chloroquine coupled with starvation for 4 h (b), 10 μg/mL SNPs for 24 h (c), 200 μg/mL SNPs for 24 h (d), 10 μg/mL SNPs for 48 h (e), or 200 μg/mL SNPs for 48 h (f). The result of western blot of various treatments on HCT-116 cells (g), GAPDH was used as a loading control, and the image was analyzed by software of AzureSpot (h). More results referring full length of blotting bands were shown in Supplementary Figure 4. These blotting bands were obtained from the same experimental conditions.
