Figure 6: SNPs induced autophagy.
From: New findings of silica nanoparticles induced ER autophagy in human colon cancer cell
Control without any treatment (a), positive control, with 12.5 μM chloroquine and starvation for 4 h (b), HCT-116 cells were treated with 10 μg/mL SNPs for 24 h (c), 200 μg/mL for 24 h (d), 10 μg/mL SNPs for 48 h (e), 200 μg/mL SNPs for 48 h (f), GFP-RFP-LC3 results analyzed by LCSM (g), and all the group under the same regulation conditions. The result of western blot of HCT-116 cells treated with 200 μg/mL SNPs for 48 h or 12.5 μM chloroquine for 1 h, then treated with 200 μg/mL SNPs for 48 h (h). Image of western blot analyzed by software of AzureSpot (i). More results referring full length of blotting bands were shown in Supplementary Figure 5. These blotting bands were obtained under the same experimental conditions.
